Dr M Manjunath Setty

Professor

Department of Pharmacognosy

CURRENT ACADEMIC ROLE & RESPONSIBILITIES

    Dr M Manjunath Setty heads the department of Pharmacognosy.

    • He takes care of all the activities related to the department.
    • He is involved in curriculum development including learner resource development and modernisation of laboratories, interaction with industry and society.
    • He provides guidance for research activities for the award of Masters and Doctor of Philosophy degrees.
    • He is involved in administration and planning both at departmental and institutional levels, and also contributes to resource mobilisation effort to the institution.
    • He participates in policy planning and evaluation as well as in promotional activities both at department and institutional levels.

 

SUBJECTS CURRENTLY TEACHING

Subject Subject code Semester
Pharmacognosy 1 PCO 106T First B Pharm
Pharmacognosy II PCO 306 Third B Pharm
Advanced Pharmacognosy and Phytochemistry PCO 601T First M Pharm

ACADEMIC QUALIFICATIONS

Degree Specialisation Institute Year of passing
PhD Manipal Academy of Higher Education 2008
M Pharm Pharmacognosy Bangalore University 1994
B Pharm Gulbarga University 1988

Experience

Institution / Organisation Designation Role Tenure
Department of Pharmacognosy, MCOPS Manipal Professor Teaching and Research March 2013 till date
Department of Pharmacognosy, MCOPS Manipal Associate Professor Teaching and Research January 2008 to March 2013
Department of Pharmacognosy, MCOPS Manipal Selection Grade Lecturer Teaching and Research September 2004 to January 2008
Department of Pharmacognosy, MCOPS Manipal Senior Lecturer Teaching and Research August 2001 to September 2004
Department of Pharmacognosy, MCOPS Manipal Lecturer Teaching and Research June 2001 to August 2001
Sree Siddaganga College of Pharmacy, Tumkur Lecturer and Head Taught D Pharm and B Pharm students. 12 year 4 months

AREAS OF INTEREST, EXPERTISE AND RESEARCH

Area of Interest

Pharmacognosy and Phytochemistry, Biological Screening of herbal drugs, Herbal Drug Formulation and Standardisation

Area of Expertise

Nephroprotective Studies, Antiurolithiatic Activity

Area of Research

Pharmacognosy and Phytochemistry, Biological screening of herbal drugs

Professional Affiliations & Contributions

  • Life Member of Indian Pharmaceutical Association, 2001.
  • BOS Member of RGUHS, Bangalore.

Pharmacognostical Standardisation and HPTLC Quantification of Gallic acid in Homonoia riparia Lour

2015-06-12 Pharmacognostical studies Seena Kanniparambil Xavier Raviraj Anand Devkar Shilpee Chaudhary Chandrashekara Shastry Shreedhara M Manjunath Setty

To establish the pharmacognostical and physicochemical standardisation parameters of whole plant of Homonoia riparia Lour. Materials and Methods: The plant was studied for the morpho-anatomical characters, standardisation parameters such as ash value, extractive value, fluorescence analysis, loss on drying, swelling index, foaming index according to Indian Pharmacopoeia and WHO guidelines. Phytochemical analysis was also performed by standard methods. Quantification of gallic acid in Homonoia riparia was carried out using HPTLC technique. Results: The detailed microscopy of root revealed the presence of cork, cork cambium, pericyclic fibres, thick walled parenchyma and starch granules. The distinguishing characters of stem are presence of sclereids, xylem, phloem, fibres. Leaf microscopy showed the presence of anomocytic stomata, bicollateral vascular bundles ensheathed by fibres. Rosette crystals are present in all the parts of the plant. Starch grains are abundantly present in root and stem but absent in leaves. Various physicochemical parameters were also determined. Phytochemical screening of the extract and HPTLC quantification of gallic acid was also performed. Conclusion: The present study provides pharmacognostical, physicochemical and phytochemical details of the whole plant of Homonoia riparia which are useful in laying down standardization and pharmacopoeia parameters.

Selective Cytotoxicity and Pro‑apoptotic Activity of Stem Bark of Wrightia tinctoria (Roxb.) R. Br. in Cancerous Cells

2015-01-10 Anti cancer Shilpee Chaudhary Raviraj Anand Devkar Deepak Bhere M Manjunath Setty K Sreedhara Ranganath Pai

To investigate the anticancer potential of stem bark of W. tinctoria and establish its phytochemical basis. Materials and Methods: The ethanol extract and subsequent fractions, petroleum ether, ethyl acetate, n‑butanol, and aqueous were prepared by standard methods. In vitro cytotoxicity was determined in MCF‑7 (breast) and HeLa (cervical) adenocarcinoma cells, and V79 (nontumor fibroblast) cells and apoptogenic activity in MCF‑7 cells by acridine orange (AO)/ ethidium bromide (EB) staining. Additionally, the antioxidant potential was evaluated using suitable methods. High‑performance thin layer chromatography (HPTLC) analysis was performed for identification of active phytoconstituents. Results: Petroleum ether and ethyl acetate fractions were most potent with IC50 values of 37.78 and 29.69 μg/ml in HeLa and 31.56 and 32.63 μg/ml in MCF‑7 cells respectively in the sulforhodamine B assay. Comparable results were obtained in HeLa cells in 3‑(4,5‑dimethylthiazolyl‑2‑yl)‑2,5‑diphenyl tetrazolium bromide assay and interestingly, the fractions were found to be safe to noncancerous fibroblast cells. Both fractions induced significant (P < 0.05) apoptotic morphological changes observed by AO/EB staining. Moreover, extract/fractions exhibited excellent inhibition of lipid peroxidation with the ethyl acetate fraction being most active (IC50: 23.40 μg/ml). HPTLC confirmed the presence of two anti‑cancer triterpenoids, lupeol, and β‑sitosterol in active fractions. Conclusion: Extract/fractions of W. tinctoria exhibit selective cytotoxicity against cancerous cells that is mediated by apoptosis. Fractions are less toxic to noncancerous cells; hence, they can be developed as safer chemopreventive agents.

Evaluation of antioxidant and anticancer activity of extract and fractions of Nardostachys jatamansi DC in breast carcinoma

2015-11-10 Anti-cancer Shilpee Chaudhary Chandrashekar KS K Sreedhara Ranganath Pai M Manjunath Setty Raviraj Anand Devkar Neetinkumar Dnyanoba Reddy Muhammed Haneefa Shoja

Background: Nardostachys jatamansi DC is a Himalayan medicinal herb that has been described in various traditional systems of medicine for its use in cancer. In view of its traditional claims, and chemical constituents, antioxidant and anticancer activities were evaluated in breast carcinoma. Methods: Petroleum ether (NJPE), methanol extract (NJM) and subsequent diethyl ether (NJDE), ethyl acetate (NJEA) and aqueous (NJAQ) fractions of roots and rhizomes of N. jatamansi were prepared. Total phenolic, flavonoid content, and antioxidant activities were determined using suitable methods. Antiproliferative activity was assessed in estrogen receptor (ER)-positive (MCF-7) and ER-negative breast carcinoma (MDA-MB-231) cells by MTT and SRB assay. Cell cycle analysis, Hoechst staining, and clonogenic assay were employed to determine the mode of antiproliferative and pro-apoptotic activity in MDA-MB-231 cells. Results: NJM/fractions exhibited prominent antioxidant activity with significant correlation between phenolic content and ABTS (IC50) scavenging (R = −0.9680, P < 0.05), and total antioxidant capacity (R = 0.8396, P > 0.05). In MTT assay, NJM exhibited the highest antiproliferative activity (IC50: 58.01 ± 6.13 and 23.83 ± 0.69 μg/mL in MCF-7 and MDA-MB-231 respectively). Among the fractions, NJPE and NJDE were found to be most potent in MCF-7 (IC50: 60.59 ± 4.78 μg/mL) and MDA-MB-231 (IC50: 25.04 ± 0.90 μg/mL) cells respectively. Statistical analyses revealed NJM and NJDE exhibited significantly higher (P < 0.05) cytotoxicity in MDA-MB-231 cells. Cell cycle analysis demonstrated that NJM, NJPE and NJEA caused G2/M arrest while NJDE caused G0/G1 phase arrest in MDA-MB-231 cells. Further, NJM/fractions induced significant (P < 0.001) cell death by apoptosis characterized by apoptotic morphological changes in Hoechst staining and inhibited long-term proliferation (P < 0.001) of MDA-MB-231 cells in clonogenic assay. Lupeol and β-sitosterol were identified as anticancer principles in NJM/fractions by HPTLC. Conclusion: Our results suggest that NJM/fractions possess significant antiproliferative potential which is mediated through cell cycle perturbation and pro-apoptotic effects in MDA-MB-231 cells. Moreover, this study highlights the antioxidant potential of NJM/fractions which can be attributed to the presence of phenols. NJDE emerged as the most potent fraction and further mechanistic and phytochemical investigations are under way to identify the active principles.

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

2015-01-02 Anti-Cancer Kumar H Mihir Savaliya Subhankar Biswas Pawan G Nayak Naseer Maliyakkal M Manjunath Setty Karthik Gourishetti K Sreedhara Ranganath Pai

Various parts of Mimusops elengi Linn. (Sapotaceae) have been used widely in traditional Indian medicine for the treatment of pain, inflammation and wounds. The study was conducted to explore the use of stem bark of M. elengi on pharmacological grounds and to evaluate the scientific basis of cytotoxic and anti-tumor activity. Extract/fractions were prepared and in vitro cytotoxicity was assessed using SRB assay. Most effective fractions were subjected to fluorescence microscopy based acridine orange/ethidium bromide (AO/EB) and Hoechst 33342 staining to determine apoptosis induction and DNA fragmentation assay. Comet and micronuclei assay were performed to assess genotoxicity. Cell cycle analysis was also performed. In vivo anti-tumor potential was evaluated by Ehrlich ascites carcinoma (EAC) model in mice. The alcoholic stem bark extract of M. elengi along with four fractions showed potential in vitro cytotoxicity in SRB assay. Of these, dichloromethane and ethyl acetate fractions were selected for further studies. The fractions revealed apoptosis inducing potential in AO/EB and Hoechst 33342 staining, which was further confirmed by DNA fragmentation assay. Genotoxic potential was revealed by comet and micronuclei assay. Fractions also exhibited specific cell cycle inhibition in G0/G1 phase. In EAC model, ethyl acetate fraction along with the standard (cisplatin) effectively reduced the increase in body weight compared to control and improved mean survival time. Both fractions were able to restore the altered hematological and biochemical parameters. Hence, M. elengi stem bark may be a possible therapeutic candidate having cytotoxic and anti-tumor potential.

Preliminary evaluation of in vitro cytotoxicity and in vivo antitumor activity of Premna herbacea Roxb. in Ehrlich ascites carcinoma model and Dalton’s lymphoma ascites model

2013-01-03 Anti-cancer Isha Dhamija Nitesh Kumar S.N. Manjula Vipan Parihar M Manjunath Setty K Sreedhara Ranganath Pai

In the present study, the root nodules of Premna herbacea Roxb. (PH) was investigated for its in vitro cytotoxicity and in vivo antitumor activity. Two extracts, aqueous and alcoholic; two fractions of alcoholic extract, ethyl acetate and butanol fractions were screened for their in vitro cytotoxicity by brine shrimp lethality (BSL) assay, trypan blue exclusion assay and MTT assay. Alcoholic extract and its ethyl acetate fraction were found to be the most effective in BSL assay, trypan blue exclusion assay. In vivo antitumor activity was screened in the Ehrlich ascites carcinoma (EAC) model and the Dalton lymphoma ascites (DLA) model. The extracts and the fractions were tested at two dosages (250 and 500 mg/kg) by intraperitoneally (i.p.) route on every alternate day upto 13th day. Cisplatin was used as positive control in both studies in single dose (day 1) 3.5 mg/kg by i.p. route. In EAC model, ascites tumor was induced by inoculating 2.5 million of EAC cells i.p. alcoholic extract at 500 mg/kg was the most effective in elevating MST, reduction in body weight in EAC induced tumor. Only the effective extract i.e., alcoholic extract were studied for hematological and antioxidant parameter. It showed a restoring effect on altered hematological parameters and a significant improvement in biochemical parameters at 250 mg/kg dose of alcoholic extract. These results explain the toxicity of 500 mg/kg might be high. In the Dalton lymphoma ascites (DLA) model, solid tumor was developed by i.m. injection of 1 million DLA cells. Both the extracts and the fractions possessed potent antitumor activity against solid tumor models by significantly reducing the solid tumor weight and volume.

Isolation, characterisation and in vitro biological screening of phytoconstituents from rhixomes of Dryopteris filix mas

2013-01-01 Jothsna Devi

2013. The study was carried out to isolate and evaluate the bioactivity of the chemical constituents from the roots of Dropteris filx mas. Bioactivity guided fractionation was done with respect BSLT in vitro i assay.

Pharmacognostical, phytochemical and anti-urolithiatic studies of Bridelia retusa Spreng. Bark

2013-01-01 Himanshu Makwana

2013. The bark of Bridelia retusa was subjected to pharmacognostical, preliminary phytochemical and antiuroliatic activity.

Invitro propagation, preliminary phytochemical and anti-radiation study of Drynaria quercifololia

2012-01-01 Pallavi Srivasava

2012. Establishment of callus culture, preliminary phytochemical screening, antioxidant and antiradiation activity of Drynaria quercifolia was studied.

Phytochmical investigation and biological evaluation of fruits of Piper attenuatum

2012-01-01 S Divya Reddy

2012 . Our studies have shown the isolation of 9 neolignans for the first time in Piper attenuatum and the same was confirmed from spectral analysis. Biological evaluation of the isolated compounds resulted in excellent unreported antioxidant activity and mild carbohydrate /glucose metabolism modulating activity, insignificant antihyperglycemic and anticancer activity.

Pharmacognostical evaluation of some Ficus species from upper Gangetic planes of India

2011-01-01 Durgesh Pratap Singh

2011. The study was focused at the Pharmacognostical evaluation viz. botanical study, determination of physicochemical parameters, phytochemical analysis and HPTLC analysis of barks of five different medicinally important Ficus species.

Isolation of chemical constituents from Withania somnifera and estimation of biological activity in-vitro

2011-01-01 Akunuri Praneetha

2011 . The study was carried out to isolate and evaluate the bioactivity of the chemical constituents from the roots of Withania somniifera. Bioactivity guided fractionation was done with respect PEP inhibition assay.

Formulation, standardisation and evaluation of modified release anti-hyperlipidemic herbal tablet dosage form

2010-01-01 Nopa Ram

2010 . Guggul is formulated into herbal dosage form, standardised and studied for anti-hyperlipidaemic activity.