Achievements:

Tushar conducted his research “An investigation of environmental influence on the growth of Burkholderia pseudomallei in the soil of South West Coast of Karnataka” from July 2016 and submitted the thesis to Manipal Academy of Higher Education in March 2020. The research was completed under the guidance of Dr. Chiranjay Mukhopadhyay, Professor Dept. of Microbiology, Kasturba Medical College, Manipal. Tushardistinguished himself in writing well-researched and interesting projects which bagged him the Senior Research Fellowship from the Indian Council of Medical Research (2018-2019) and the European Molecular Biology Organization (EMBO) Short Term Fellowship. This gave him the opportunity to work in an international laboratory setup at Medical University of Graz, where he further developed his skills in whole genome sequencing and microbiome analysis. During the tenure of his research he actively participated at various National and International Conferences receiving commendable response from global experts working on the disease. Published his research in peer reviewed journals.

Tushar has recently joined the Ramaiah University of Applied Sciences where he is establishing the laboratory to continue my work on the emerging infectious diseases.

List of Publications:

1.      Shaw T, Tellapragada C , Kamath A, Eshwara VK, Mukhopadhyay C. Implications of environmental and pathogen- specific determinants on clinical presentations and disease outcome in melioidosis patients. PLoSNegl Trop Dis. 2019; 1–13.

2.      Kamthan A, Shaw T, Mukhopadhyay C, Kumar S. Molecular analysis of clinical Burkholderia pseudomallei isolates from southwestern coastal region of India, using multi-locus sequence typing. PLoSNegl Trop Dis. 2018; 1–12.

3.      Mukhopadhyay C, Shaw T, Varghese G, Dance D. Melioidosis in South Asia (India, Nepal, Pakistan, Bhutan and Afghanistan). Trop Med Infect Dis. 2018; 3(2):51. 

4.      Shaw T, Tellapragada C, Vandana KE, AuCoin DP, Mukhopadhyay C. Performance evaluation of active melioidosis detect-lateral flow assay (AMD-LFA) for diagnosis of melioidosis in endemic settings with limited resources. PLoS One. 2018; 13(3):1–7

Conference Presentation:

1.      Novel allelic profile of the clinical strains of Burkholderia pseudomallei on multi locus sequence typing from India, Poster Presentation, 17^th International Conference on Infectious Diseases, Hyderabad, India 2016

2.      Soil epidemiology in South West India. Oral Presentation, 2^nd South Asian Melioidosis Congress, Colombo, Sri Lanka, 2018

3.      Determining the influence of environmental factors on the presence of B. pseudomallei in the Indian soil. Oral Presentation, 9^th  World Melioidosis Congress, Hanoi, Vietnam, 2019 

Academic Qualification

PhD Biochemistry, Kasturba Medical College, Manipal. (Feb 2021)

MSc Medical Biochemistry, Kasturba Medical College, Manipal. (Aug 2009)

BSc Biotechnology, Botany, Chemistry,

Sahyadri Science College Shimoga (May 2006)

Present Status

Research Consultant, Microbiology & Molecular Biology Lab, Bio Edge Solutions, Bengaluru.

Research Consultant, Indus Group of Institutions, Mangalore.

Achievements

Dr. Shetty conducted her research, “Exploring the feasibility of Newborn Screening Program (NBS) for metabolic disorders in Udupi District” from Jul 2017 to Jul 2020 under the guidance of Dr. Nalini K, Former Professor, Department of Biochemistry and Dr. Leslie Lewis, Professor & Head, Department of Pediatrics, Kasturba Medical College, Manipal. She was awarded PhD by Manipal Academy of Higher Education on Feb 2021. Her work was funded by 2 extra mural grants; Grand Challenges Canada, Canada and VGST, Karnataka outcome and are as follows.

1.      Approx. 22 % of total live-births of district benefited.

2.      Established the Incidence data of IEM at Udupi.

3.      Successful district wide initiation of NBS at government hospitals and Manipal hospitals

4.      Positive acknowledgement for the state wide NBS from State authority

Publications: Total 8

·  Shetty, PP, et al, Reverse Phase-High Performance Liquid Chromatography: An Alternative to Expensive Tandem Mass Spectrometry Screening for Amino Acid Profiling in Dried Blood Spot in Resource Constrained Diagnostic Settings. JKIMSU. July-September 2021; 10 (3), 29-39.

·  Shetty, PP, Thriveni, Nalini K. Inhibition assay of ala dehydratase by succinyl acetone in tyrosinemia type 1: a rapid ht1 screening technique for resource constrained diagnostic setups. Malaysian Journal of Biochemistry and Molecular Biology 2020; (3), 66-70.

·  Shetty, PP., Nagendra, K., Nalini, K., Rao P, Bhaskaranand. N, Belle V. S. Amino Acid Profile and Lactate Pyruvate Ratio: Potential Adjunct Markers for Differentiating Inborn Errors of Metabolism. Ind J Clin Biochem (2019). https://doi.org/10.1007/s12291-019-00840-1

• Vemugadda, H., Shetty, PP., Biswas, M., Shenoy, R. P., & Nalini, K. Erythrocyte arginase activity and serum nitric oxide in diabetes mellitus. Malaysian Journal of Biochemistry and Molecular Biology.2019; 22(3), 33-37.

Scientific proposals for research granting bodies:  Total 8

Conference Presentations: Total- 9

●   ICHTR-2019. 29^th – 31^st Oct, 2019. Awareness and perception among Indian mothers on neonatal screening for congenital and metabolic disorders (oral) - best oral presentation awarded under Health Science category.

●  DIAGNOCON. Feb 23^rd to 25^th 2018. Manipal. Thin Layer Chromatography –Noninvasive method for Screening Alkaptonuria, 2nd prize for the best poster award

●  IEMBIOCON 7^th April 2016. Chennai. Aminogram and LP ratio- A marker for differentiating IEM disorders, 1st prize for the best e-poster award (co-author)

Thesis:

My thesis titled “Evaluation of genetic factors responsible for lethal human fetal malformations” under the guidance of Prof. Dr Girisha KM, Department of Medical Genetics, Kasturba Medical College, Manipal. Our work delineates the phenotypic spectrum of various congenital fetal malformation syndromes and deciphering genomic alterations responsible for lethal fetal malformations.

Education and training:

·         Ph.D in Medical Genetics from Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Karnataka, India (Thesis submitted on July 2020; tentative graduation August 2020)

·         M.Sc. in Medical Anatomy (2010-2013) from Kasturba Medical College, Manipal, Manipal Academy of Higher Education Karnataka, India.

·         Bachelor of Physiotherapy (2004-2009) fromThe Tamil Nadu Dr. M.G.R. Medical University, Chennai, Tamil Nadu,India.

·         Certificate course in “Biostatistics, Epidemiology and Research Methodology” and “Bioethics” in Manipal Academy of Higher Education, Karnataka, India.

Selected publications:

1.       Chong JX, Talbot JC, Teets EM, Previs S, Martin BL, Shively KM,Marvin CT, Aylsworth AS, Saadeh-Haddad R, Schatz UA, Inzana F, Ben-Omran T, Almusafri F, Al-Mulla M, Buckingham KJ, Harel T, Mor-Shaked H, Radhakrishnan P, Girisha KM, Nayak SS, Shukla A, Dieterich K, Faure J, Rendu J,  Capri Y, Latypova X, Nickerson DA,Warshaw D, Janssen PM, University of Washington Center for Mendelian Genomics, Amacher SL, Bamshad MJ. Mutations in MYLPF cause a novel segmental amyoplasia that manifests as distal arthrogryposis. American Journal of Human Genetics. 2020 Aug 6;107(2):293-310.). Impact factor: 9.924

2.       Radhakrishnan P, Nayak SS, Shukla A, Girisha KM. Biallelic c. 1263dupC in DOK7 results in fetal akinesia deformation sequence. American Journal of Medical Genetics Part A. 2020 Apr;182(4):804-7.Impact factor: 2.125

3.       Radhakrishnan P, Nayak SS, Shukla A, Girisha KM. Clinical and mutation profile in six fetuses. Clinical genetics. 2019 Dec;96(6):560-5.Impact factor:4.104

4.       Pehlivan D, Bayram Y, Gunes N, Coban Akdemir Z, Shukla A, Bierhals T, Tabakci B, Sahin Y, Gezdirici A, Fatih JM, Gulec EY, Yesil G, Punetha J, Ocak Z, Grochowski CM, Karaca E, Albayrak HM, Radhakrishnan P, Erdem HB, Sahin I, Yildirim T, Bayhan IA, Bursali A, Elmas M, Yuksel Z, Ozdemir O, Silan F, Yildiz O, Yesilbas O, Isikay S, Balta B, Gu S, Jhangiani SN, Doddapaneni H, Hu J, Muzny DM; Baylor-Hopkins Center for Mendelian Genomics, Boerwinkle E, Gibbs RA, Tsiakas K, Hempel M, Girisha KM, Gul D, Posey JE, Elcioglu NH, Tuysuz B, Lupski JR. The Genomics of Arthrogryposis, a Complex Trait: Candidate Genes and Further Evidence for Oligogenic Inheritance. American Journal of Human Genetics. 2019 Jul 3;105(1):132-150.Impact factor: 9.924