Assisted reproduction is mainly limited by low success rates and a high rate of multiple pregnancies. Limitations in morphology based embryo selection methods have driven the search for adjunctive technologies and metabolic profiling of embryo culture media has emerged as one among the promising techniques to predict embryo viability and genetic integrity. This study is focused on the utilization of spent embryo culture medium to non invasively detect the viability and quality of the pre implantation embryos. Mouse embryo metabolism is being studied non invasively by Nuclear Magnetic Resonance (NMR) spectroscopy based analysis of embryo culture media. The effect of all factors affecting embryo quality like sperm quality, oocyte quality and the in vitro culture conditions will be taken into account. The change in embryo metabolism with respect to these factors will be evaluated both non invasively by NMR and by invasive methods in order to establish a unique biomarker that can predict embryo developmental potential, implantation potential as well as genetic integrity. The results from this study are expected to provide valuable information which help in improving embryo selection techniques, enhance the chances of conception and reduce complications associated with multiple gestations. (Supported by Indian Council of Medical Research, Govt. of India)